Construct: shRNA TRCN0000167054

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032451.1-2826s1c1
DNA Barcode:
CATGATGAAATGTTGTCTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPIRE2 (84501)
Gene Description:
spire type actin nucleation factor 2
Transcript:
RefSeq NM_032451.1 (NON-CURRENT)
Match location:
Position 2826 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000167054)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGATGAAATGTTGTCTCTA
Hairpin Sequence:
5'-CCGG-CATGATGAAATGTTGTCTCTA-CTCGAG-TAGAGACAACATTTCATCATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGATGAAATGTTGTCTCTACTCGAGTAGAGACAACATTTCATCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGATGAAATGTTGTCTCTACTCGAGTAGAGACAACATTTCATCATG-3'

Other clones with same target sequence:

(none)