Construct: shRNA TRCN0000167207

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022101.2-691s1c1
DNA Barcode:
CTTGATTGACAACCAGTTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CXorf56 (63932)
Gene Description:
chromosome X open reading frame 56
Transcript:
RefSeq NM_022101.2 (NON-CURRENT)
Match location:
Position 691 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000167207)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGATTGACAACCAGTTCAA
Hairpin Sequence:
5'-CCGG-CTTGATTGACAACCAGTTCAA-CTCGAG-TTGAACTGGTTGTCAATCAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGATTGACAACCAGTTCAACTCGAGTTGAACTGGTTGTCAATCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGATTGACAACCAGTTCAACTCGAGTTGAACTGGTTGTCAATCAAG-3'

Other clones with same target sequence:

(none)