Construct: shRNA TRCN0000167417

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031941.2-1031s1c1
DNA Barcode:
GCTCAAATGCTTTAATCGTCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
USHBP1 (83878)
Gene Description:
USH1 protein network component harmonin binding protein 1
Transcript:
RefSeq NM_031941.2 (NON-CURRENT)
Match location:
Position 1031 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000167417)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAAATGCTTTAATCGTCT
Hairpin Sequence:
5'-CCGG-GCTCAAATGCTTTAATCGTCT-CTCGAG-AGACGATTAAAGCATTTGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAAATGCTTTAATCGTCTCTCGAGAGACGATTAAAGCATTTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAAATGCTTTAATCGTCTCTCGAGAGACGATTAAAGCATTTGAGC-3'

Other clones with same target sequence:

(none)