Construct: shRNA TRCN0000167419

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032860.3-1327s1c1
DNA Barcode:
GCAGTGATCTTCCTAAAGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LTV1 (84946)
Gene Description:
LTV1 ribosome biogenesis factor
Transcript:
RefSeq NM_032860.3 (NON-CURRENT)
Match location:
Position 1327 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000167419)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTGATCTTCCTAAAGTAT
Hairpin Sequence:
5'-CCGG-GCAGTGATCTTCCTAAAGTAT-CTCGAG-ATACTTTAGGAAGATCACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTGATCTTCCTAAAGTATCTCGAGATACTTTAGGAAGATCACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTGATCTTCCTAAAGTATCTCGAGATACTTTAGGAAGATCACTGC-3'

Other clones with same target sequence:

(none)