Construct: shRNA TRCN0000167422

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145235.2-1080s1c1
DNA Barcode:
GTAGTCTCCTTATTAGAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FANK1 (92565)
Gene Description:
fibronectin type III and ankyrin repeat domains 1
Transcript:
RefSeq NM_145235.2 (NON-CURRENT)
Match location:
Position 1080 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000167422)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGTCTCCTTATTAGAAGAA
Hairpin Sequence:
5'-CCGG-GTAGTCTCCTTATTAGAAGAA-CTCGAG-TTCTTCTAATAAGGAGACTAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGTCTCCTTATTAGAAGAACTCGAGTTCTTCTAATAAGGAGACTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGTCTCCTTATTAGAAGAACTCGAGTTCTTCTAATAAGGAGACTAC-3'

Other clones with same target sequence:

(none)