Construct: shRNA TRCN0000167448

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016052.2-1132s1c1
DNA Barcode:
GTCATGTCTATTGGTGTATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RRP15 (51018)
Gene Description:
ribosomal RNA processing 15 homolog
Transcript:
RefSeq NM_016052.2 (NON-CURRENT)
Match location:
Position 1132 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000167448)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCATGTCTATTGGTGTATTA
Hairpin Sequence:
5'-CCGG-GTCATGTCTATTGGTGTATTA-CTCGAG-TAATACACCAATAGACATGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCATGTCTATTGGTGTATTACTCGAGTAATACACCAATAGACATGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCATGTCTATTGGTGTATTACTCGAGTAATACACCAATAGACATGAC-3'

Other clones with same target sequence:

(none)