Construct: shRNA TRCN0000167454

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024688.1-1638s1c1
DNA Barcode:
GAGACTGATAAGAACTTCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC7 (79741)
Gene Description:
coiled-coil domain containing 7
Transcript:
RefSeq NM_024688.1 (NON-CURRENT)
Match location:
Position 1638 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000167454)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGACTGATAAGAACTTCTTT
Hairpin Sequence:
5'-CCGG-GAGACTGATAAGAACTTCTTT-CTCGAG-AAAGAAGTTCTTATCAGTCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGACTGATAAGAACTTCTTTCTCGAGAAAGAAGTTCTTATCAGTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGACTGATAAGAACTTCTTTCTCGAGAAAGAAGTTCTTATCAGTCTC-3'

Other clones with same target sequence:

(none)