Construct: shRNA TRCN0000167507

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032320.4-1769s1c1
DNA Barcode:
GCACATAACAATGTTTAGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BTBD10 (84280)
Gene Description:
BTB domain containing 10
Transcript:
RefSeq NM_032320.4 (NON-CURRENT)
Match location:
Position 1769 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000167507)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACATAACAATGTTTAGGAT
Hairpin Sequence:
5'-CCGG-GCACATAACAATGTTTAGGAT-CTCGAG-ATCCTAAACATTGTTATGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACATAACAATGTTTAGGATCTCGAGATCCTAAACATTGTTATGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACATAACAATGTTTAGGATCTCGAGATCCTAAACATTGTTATGTGC-3'

Other clones with same target sequence:

(none)