Construct: shRNA TRCN0000167543

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001007542.1-1101s1c1
DNA Barcode:
GAGTAGAAGATACATGAGGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FLJ40453 (401217)
Gene Description:
hypothetical LOC401217
Transcript:
RefSeq NM_001007542.1 (NON-CURRENT)
Match location:
Position 1101 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000167543)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTAGAAGATACATGAGGTT
Hairpin Sequence:
5'-CCGG-GAGTAGAAGATACATGAGGTT-CTCGAG-AACCTCATGTATCTTCTACTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTAGAAGATACATGAGGTTCTCGAGAACCTCATGTATCTTCTACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTAGAAGATACATGAGGTTCTCGAGAACCTCATGTATCTTCTACTC-3'

Other clones with same target sequence:

(none)