Construct: shRNA TRCN0000167579

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019051.1-299s1c1
DNA Barcode:
GATAGTCGTCTAAAGTTCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MRPL50 (54534)
Gene Description:
mitochondrial ribosomal protein L50
Transcript:
RefSeq NM_019051.1 (NON-CURRENT)
Match location:
Position 299 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000167579)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATAGTCGTCTAAAGTTCAAT
Hairpin Sequence:
5'-CCGG-GATAGTCGTCTAAAGTTCAAT-CTCGAG-ATTGAACTTTAGACGACTATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATAGTCGTCTAAAGTTCAATCTCGAGATTGAACTTTAGACGACTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATAGTCGTCTAAAGTTCAATCTCGAGATTGAACTTTAGACGACTATC-3'

Other clones with same target sequence:

(none)