Construct: shRNA TRCN0000167653

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153354.2-2183s1c1
DNA Barcode:
GCTTGAGAATAGCAAGAGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM161B (153396)
Gene Description:
transmembrane protein 161B
Transcript:
RefSeq NM_153354.2 (NON-CURRENT)
Match location:
Position 2183 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000167653)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGAGAATAGCAAGAGTAT
Hairpin Sequence:
5'-CCGG-GCTTGAGAATAGCAAGAGTAT-CTCGAG-ATACTCTTGCTATTCTCAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGAGAATAGCAAGAGTATCTCGAGATACTCTTGCTATTCTCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGAGAATAGCAAGAGTATCTCGAGATACTCTTGCTATTCTCAAGC-3'

Other clones with same target sequence:

(none)