Construct: shRNA TRCN0000167694

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001732.1-854s1c1
DNA Barcode:
GACGGAGATATTAAGATTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CC2D2B (387707)
Gene Description:
coiled-coil and C2 domain containing 2B
Transcript:
RefSeq NM_001001732.1 (NON-CURRENT)
Match location:
Position 854 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000167694)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGGAGATATTAAGATTCTT
Hairpin Sequence:
5'-CCGG-GACGGAGATATTAAGATTCTT-CTCGAG-AAGAATCTTAATATCTCCGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGGAGATATTAAGATTCTTCTCGAGAAGAATCTTAATATCTCCGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGGAGATATTAAGATTCTTCTCGAGAAGAATCTTAATATCTCCGTC-3'

Other clones with same target sequence:

(none)