Construct: shRNA TRCN0000167763

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025145.3-2700s1c1
DNA Barcode:
GCAAAGATGATAAAGGATGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CFAP43 (80217)
Gene Description:
cilia and flagella associated protein 43
Transcript:
RefSeq NM_025145.3 (NON-CURRENT)
Match location:
Position 2700 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000167763)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAAGATGATAAAGGATGTA
Hairpin Sequence:
5'-CCGG-GCAAAGATGATAAAGGATGTA-CTCGAG-TACATCCTTTATCATCTTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAAGATGATAAAGGATGTACTCGAGTACATCCTTTATCATCTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAAGATGATAAAGGATGTACTCGAGTACATCCTTTATCATCTTTGC-3'

Other clones with same target sequence:

(none)