Construct: shRNA TRCN0000167773

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153261.3-132s1c1
DNA Barcode:
GAGGAGACTTACTGAATATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CNEP1R1 (255919)
Gene Description:
CTD nuclear envelope phosphatase 1 regulatory subunit 1
Transcript:
RefSeq NM_153261.3 (NON-CURRENT)
Match location:
Position 132 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000167773)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGAGACTTACTGAATATAT
Hairpin Sequence:
5'-CCGG-GAGGAGACTTACTGAATATAT-CTCGAG-ATATATTCAGTAAGTCTCCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGAGACTTACTGAATATATCTCGAGATATATTCAGTAAGTCTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGAGACTTACTGAATATATCTCGAGATATATTCAGTAAGTCTCCTC-3'

Other clones with same target sequence:

(none)