Construct: shRNA TRCN0000167922

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032134.1-2809s1c1
DNA Barcode:
CCACCAGAAACTTATCAGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
QRICH2 (84074)
Gene Description:
glutamine rich 2
Transcript:
RefSeq NM_032134.1 (NON-CURRENT)
Match location:
Position 2809 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000167922)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCAGAAACTTATCAGCAA
Hairpin Sequence:
5'-CCGG-CCACCAGAAACTTATCAGCAA-CTCGAG-TTGCTGATAAGTTTCTGGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCAGAAACTTATCAGCAACTCGAGTTGCTGATAAGTTTCTGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCAGAAACTTATCAGCAACTCGAGTTGCTGATAAGTTTCTGGTGG-3'

Other clones with same target sequence:

(none)