Construct: shRNA TRCN0000167941

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001025202.1-784s1c1
DNA Barcode:
CGCTGCTTACTTAGTAGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STAG3L2 (442582)
Gene Description:
stromal antigen 3-like 2 (pseudogene)
Transcript:
RefSeq NM_001025202.1 (NON-CURRENT)
Match location:
Position 784 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000167941)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTGCTTACTTAGTAGACAA
Hairpin Sequence:
5'-CCGG-CGCTGCTTACTTAGTAGACAA-CTCGAG-TTGTCTACTAAGTAAGCAGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTGCTTACTTAGTAGACAACTCGAGTTGTCTACTAAGTAAGCAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTGCTTACTTAGTAGACAACTCGAGTTGTCTACTAAGTAAGCAGCG-3'

Other clones with same target sequence:

  1. TRCN0000062690