Construct: shRNA TRCN0000168076

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024646.1-638s1c1
DNA Barcode:
CGAGCTTTAAGCATCACGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZYG11B (79699)
Gene Description:
zyg-11 family member B, cell cycle regulator
Transcript:
RefSeq NM_024646.1 (NON-CURRENT)
Match location:
Position 638 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000168076)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGCTTTAAGCATCACGAAT
Hairpin Sequence:
5'-CCGG-CGAGCTTTAAGCATCACGAAT-CTCGAG-ATTCGTGATGCTTAAAGCTCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGCTTTAAGCATCACGAATCTCGAGATTCGTGATGCTTAAAGCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGCTTTAAGCATCACGAATCTCGAGATTCGTGATGCTTAAAGCTCG-3'

Other clones with same target sequence:

(none)