Construct: shRNA TRCN0000168163

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207350.1-495s1c1
DNA Barcode:
CAGCTTGATACGTTGGAATCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FRG1BP (284802)
Gene Description:
FSHD region gene 1 family member B, pseudogene
Transcript:
RefSeq NM_207350.1 (NON-CURRENT)
Match location:
Position 495 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000168163)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCTTGATACGTTGGAATCT
Hairpin Sequence:
5'-CCGG-CAGCTTGATACGTTGGAATCT-CTCGAG-AGATTCCAACGTATCAAGCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCTTGATACGTTGGAATCTCTCGAGAGATTCCAACGTATCAAGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCTTGATACGTTGGAATCTCTCGAGAGATTCCAACGTATCAAGCTG-3'

Other clones with same target sequence:

(none)