Construct: shRNA TRCN0000168187

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152470.2-4864s1c1
DNA Barcode:
CGTTGCTAGTTTACAGGGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNF165 (494470)
Gene Description:
ring finger protein 165
Transcript:
RefSeq NM_152470.2 (NON-CURRENT)
Match location:
Position 4864 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000168187)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTGCTAGTTTACAGGGTTT
Hairpin Sequence:
5'-CCGG-CGTTGCTAGTTTACAGGGTTT-CTCGAG-AAACCCTGTAAACTAGCAACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTGCTAGTTTACAGGGTTTCTCGAGAAACCCTGTAAACTAGCAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTGCTAGTTTACAGGGTTTCTCGAGAAACCCTGTAAACTAGCAACG-3'

Other clones with same target sequence:

(none)