Construct: shRNA TRCN0000168378

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152721.1-655s1c1
DNA Barcode:
GCTTGACTGAACCAATGACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DOK6 (220164)
Gene Description:
docking protein 6
Transcript:
RefSeq NM_152721.1 (NON-CURRENT)
Match location:
Position 655 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000168378)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGACTGAACCAATGACAT
Hairpin Sequence:
5'-CCGG-GCTTGACTGAACCAATGACAT-CTCGAG-ATGTCATTGGTTCAGTCAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGACTGAACCAATGACATCTCGAGATGTCATTGGTTCAGTCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGACTGAACCAATGACATCTCGAGATGTCATTGGTTCAGTCAAGC-3'

Other clones with same target sequence:

(none)