Construct: shRNA TRCN0000168379

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153040.1-866s1c1
DNA Barcode:
GCAGATATTCTGTGTAGCTGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C15orf32 (145858)
Gene Description:
chromosome 15 open reading frame 32
Transcript:
RefSeq NM_153040.1 (NON-CURRENT)
Match location:
Position 866 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000168379)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATATTCTGTGTAGCTGA
Hairpin Sequence:
5'-CCGG-GCAGATATTCTGTGTAGCTGA-CTCGAG-TCAGCTACACAGAATATCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATATTCTGTGTAGCTGACTCGAGTCAGCTACACAGAATATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATATTCTGTGTAGCTGACTCGAGTCAGCTACACAGAATATCTGC-3'

Other clones with same target sequence:

(none)