Construct: shRNA TRCN0000168398

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153773.1-424s1c1
DNA Barcode:
GAACACCTGACGAATAGGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKRD30BP2 (149992)
Gene Description:
ankyrin repeat domain 30B pseudogene 2
Transcript:
RefSeq NM_153773.1 (NON-CURRENT)
Match location:
Position 424 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000168398)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACACCTGACGAATAGGATA
Hairpin Sequence:
5'-CCGG-GAACACCTGACGAATAGGATA-CTCGAG-TATCCTATTCGTCAGGTGTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACACCTGACGAATAGGATACTCGAGTATCCTATTCGTCAGGTGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACACCTGACGAATAGGATACTCGAGTATCCTATTCGTCAGGTGTTC-3'

Other clones with same target sequence:

(none)