Construct: shRNA TRCN0000168495

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017999.3-3199s1c1
DNA Barcode:
GCACACTACAAAGAGTATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNF31 (55072)
Gene Description:
ring finger protein 31
Transcript:
RefSeq NM_017999.3 (NON-CURRENT)
Match location:
Position 3199 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000168495)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACACTACAAAGAGTATCTT
Hairpin Sequence:
5'-CCGG-GCACACTACAAAGAGTATCTT-CTCGAG-AAGATACTCTTTGTAGTGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACACTACAAAGAGTATCTTCTCGAGAAGATACTCTTTGTAGTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACACTACAAAGAGTATCTTCTCGAGAAGATACTCTTTGTAGTGTGC-3'

Other clones with same target sequence:

(none)