Construct: shRNA TRCN0000168574

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032809.2-1279s1c1
DNA Barcode:
GAAGACAAGAGTAACCAGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MIGA2 (84895)
Gene Description:
mitoguardin 2
Transcript:
RefSeq NM_032809.2 (NON-CURRENT)
Match location:
Position 1279 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000168574)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGACAAGAGTAACCAGCTT
Hairpin Sequence:
5'-CCGG-GAAGACAAGAGTAACCAGCTT-CTCGAG-AAGCTGGTTACTCTTGTCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGACAAGAGTAACCAGCTTCTCGAGAAGCTGGTTACTCTTGTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGACAAGAGTAACCAGCTTCTCGAGAAGCTGGTTACTCTTGTCTTC-3'

Other clones with same target sequence:

(none)