Construct: shRNA TRCN0000168609

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201400.1-765s1c1
DNA Barcode:
GATGTTGTCATTGCAGCAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EEF2KMT (196483)
Gene Description:
eukaryotic elongation factor 2 lysine methyltransferase
Transcript:
RefSeq NM_201400.1 (NON-CURRENT)
Match location:
Position 765 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000168609)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGTTGTCATTGCAGCAGAT
Hairpin Sequence:
5'-CCGG-GATGTTGTCATTGCAGCAGAT-CTCGAG-ATCTGCTGCAATGACAACATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGTTGTCATTGCAGCAGATCTCGAGATCTGCTGCAATGACAACATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGTTGTCATTGCAGCAGATCTCGAGATCTGCTGCAATGACAACATC-3'

Other clones with same target sequence:

(none)