Construct: shRNA TRCN0000168616

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014039.2-872s1c1
DNA Barcode:
GAAGGTGGACACTACCATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C11orf54 (28970)
Gene Description:
chromosome 11 open reading frame 54
Transcript:
RefSeq NM_014039.2 (NON-CURRENT)
Match location:
Position 872 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000168616)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGTGGACACTACCATTAT
Hairpin Sequence:
5'-CCGG-GAAGGTGGACACTACCATTAT-CTCGAG-ATAATGGTAGTGTCCACCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGTGGACACTACCATTATCTCGAGATAATGGTAGTGTCCACCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGTGGACACTACCATTATCTCGAGATAATGGTAGTGTCCACCTTC-3'

Other clones with same target sequence:

(none)