Construct: shRNA TRCN0000168652

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017749.2-4825s1c1
DNA Barcode:
GCATGTGGACTCTTAACTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AMBRA1 (55626)
Gene Description:
autophagy and beclin 1 regulator 1
Transcript:
RefSeq NM_017749.2 (NON-CURRENT)
Match location:
Position 4825 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000168652)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGTGGACTCTTAACTGTA
Hairpin Sequence:
5'-CCGG-GCATGTGGACTCTTAACTGTA-CTCGAG-TACAGTTAAGAGTCCACATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGTGGACTCTTAACTGTACTCGAGTACAGTTAAGAGTCCACATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGTGGACTCTTAACTGTACTCGAGTACAGTTAAGAGTCCACATGC-3'

Other clones with same target sequence:

(none)