Construct: shRNA TRCN0000168655

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018376.2-371s1c1
DNA Barcode:
GAAAGCCTTAGCCAACTGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NIPSNAP3B (55335)
Gene Description:
nipsnap homolog 3B
Transcript:
RefSeq NM_018376.2 (NON-CURRENT)
Match location:
Position 371 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000168655)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGCCTTAGCCAACTGTAA
Hairpin Sequence:
5'-CCGG-GAAAGCCTTAGCCAACTGTAA-CTCGAG-TTACAGTTGGCTAAGGCTTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGCCTTAGCCAACTGTAACTCGAGTTACAGTTGGCTAAGGCTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGCCTTAGCCAACTGTAACTCGAGTTACAGTTGGCTAAGGCTTTC-3'

Other clones with same target sequence:

(none)