Construct: shRNA TRCN0000168660

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024563.2-692s1c1
DNA Barcode:
GAAGAGAGAAATGCATGGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C5orf23 (79614)
Gene Description:
chromosome 5 open reading frame 23
Transcript:
RefSeq NM_024563.2 (NON-CURRENT)
Match location:
Position 692 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000168660)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAGAGAAATGCATGGGAA
Hairpin Sequence:
5'-CCGG-GAAGAGAGAAATGCATGGGAA-CTCGAG-TTCCCATGCATTTCTCTCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAGAGAAATGCATGGGAACTCGAGTTCCCATGCATTTCTCTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAGAGAAATGCATGGGAACTCGAGTTCCCATGCATTTCTCTCTTC-3'

Other clones with same target sequence:

(none)