Construct: shRNA TRCN0000168792

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001012626.1-286s1c1
DNA Barcode:
GCTGAATTATCAGGGACACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC285074 (285074)
Gene Description:
anaphase promoting complex subunit 1 pseudogene
Transcript:
RefSeq NM_001012626.1 (NON-CURRENT)
Match location:
Position 286 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000168792)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAATTATCAGGGACACTT
Hairpin Sequence:
5'-CCGG-GCTGAATTATCAGGGACACTT-CTCGAG-AAGTGTCCCTGATAATTCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAATTATCAGGGACACTTCTCGAGAAGTGTCCCTGATAATTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAATTATCAGGGACACTTCTCGAGAAGTGTCCCTGATAATTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000033913