Construct: shRNA TRCN0000172232

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001025463.1-231s1c1
DNA Barcode:
CAAGGGTGTCCTTGTGTCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LINC01270 (284751)
Gene Description:
long intergenic non-protein coding RNA 1270
Transcript:
RefSeq NM_001025463.1 (NON-CURRENT)
Match location:
Position 231 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000172232)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGGGTGTCCTTGTGTCTTT
Hairpin Sequence:
5'-CCGG-CAAGGGTGTCCTTGTGTCTTT-CTCGAG-AAAGACACAAGGACACCCTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGGGTGTCCTTGTGTCTTTCTCGAGAAAGACACAAGGACACCCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGGGTGTCCTTGTGTCTTTCTCGAGAAAGACACAAGGACACCCTTG-3'

Other clones with same target sequence:

(none)