Construct: shRNA TRCN0000172312

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201400.1-633s1c1
DNA Barcode:
CGAGGGAATGTCCTTCTCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EEF2KMT (196483)
Gene Description:
eukaryotic elongation factor 2 lysine methyltransferase
Transcript:
RefSeq NM_201400.1 (NON-CURRENT)
Match location:
Position 633 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000172312)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGGGAATGTCCTTCTCAAT
Hairpin Sequence:
5'-CCGG-CGAGGGAATGTCCTTCTCAAT-CTCGAG-ATTGAGAAGGACATTCCCTCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGGGAATGTCCTTCTCAATCTCGAGATTGAGAAGGACATTCCCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGGGAATGTCCTTCTCAATCTCGAGATTGAGAAGGACATTCCCTCG-3'

Other clones with same target sequence:

(none)