Construct: shRNA TRCN0000172349
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_032937.2-758s1c1
- DNA Barcode:
- CCATCTCCGTTGTTTCTGGAA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- ARRDC1-AS1 (85026)
- Gene Description:
- ARRDC1 antisense RNA 1
- Transcript:
- RefSeq NM_032937.2 (NON-CURRENT)
- Match location:
- Position 758 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000172349)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
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Sequence Information
- Target Sequence:
- CCATCTCCGTTGTTTCTGGAA
- Hairpin Sequence:
- 5'-CCGG-CCATCTCCGTTGTTTCTGGAA-CTCGAG-TTCCAGAAACAACGGAGATGG-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCATCTCCGTTGTTTCTGGAACTCGAGTTCCAGAAACAACGGAGATGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCATCTCCGTTGTTTCTGGAACTCGAGTTCCAGAAACAACGGAGATGG-3'