Construct: shRNA TRCN0000172511

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024035.1-1664s1c1
DNA Barcode:
CCAAACCCTCTTAGCAGTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RHPN1-AS1 (78998)
Gene Description:
RHPN1 antisense RNA 1 (head to head)
Transcript:
RefSeq NM_024035.1 (NON-CURRENT)
Match location:
Position 1664 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000172511)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAACCCTCTTAGCAGTCTT
Hairpin Sequence:
5'-CCGG-CCAAACCCTCTTAGCAGTCTT-CTCGAG-AAGACTGCTAAGAGGGTTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAACCCTCTTAGCAGTCTTCTCGAGAAGACTGCTAAGAGGGTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAACCCTCTTAGCAGTCTTCTCGAGAAGACTGCTAAGAGGGTTTGG-3'

Other clones with same target sequence:

(none)