Construct: shRNA TRCN0000172580

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024296.2-621s1c1
DNA Barcode:
GCAATCTGGAAGACCTCAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC28B (79140)
Gene Description:
coiled-coil domain containing 28B
Transcript:
RefSeq NM_024296.2 (NON-CURRENT)
Match location:
Position 621 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000172580)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATCTGGAAGACCTCAGTA
Hairpin Sequence:
5'-CCGG-GCAATCTGGAAGACCTCAGTA-CTCGAG-TACTGAGGTCTTCCAGATTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATCTGGAAGACCTCAGTACTCGAGTACTGAGGTCTTCCAGATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATCTGGAAGACCTCAGTACTCGAGTACTGAGGTCTTCCAGATTGC-3'

Other clones with same target sequence:

(none)