Construct: shRNA TRCN0000172606

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001013464.1-1716s1c1
DNA Barcode:
GCCAATGTTCCTTCGGCATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC441242 (441242)
Gene Description:
uncharacterized LOC441242
Transcript:
RefSeq NM_001013464.1 (NON-CURRENT)
Match location:
Position 1716 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000172606)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAATGTTCCTTCGGCATTA
Hairpin Sequence:
5'-CCGG-GCCAATGTTCCTTCGGCATTA-CTCGAG-TAATGCCGAAGGAACATTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAATGTTCCTTCGGCATTACTCGAGTAATGCCGAAGGAACATTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAATGTTCCTTCGGCATTACTCGAGTAATGCCGAAGGAACATTGGC-3'

Other clones with same target sequence:

(none)