Construct: shRNA TRCN0000172657

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017907.1-511s1c1
DNA Barcode:
GCTGGTTGTACAGTTTGGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LAMTOR1 (55004)
Gene Description:
late endosomal/lysosomal adaptor, MAPK and MTOR activator 1
Transcript:
RefSeq NM_017907.1 (NON-CURRENT)
Match location:
Position 511 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000172657)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGTTGTACAGTTTGGGAT
Hairpin Sequence:
5'-CCGG-GCTGGTTGTACAGTTTGGGAT-CTCGAG-ATCCCAAACTGTACAACCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGTTGTACAGTTTGGGATCTCGAGATCCCAAACTGTACAACCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGTTGTACAGTTTGGGATCTCGAGATCCCAAACTGTACAACCAGC-3'

Other clones with same target sequence:

(none)