Construct: shRNA TRCN0000172723

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207477.1-1782s1c1
DNA Barcode:
GCCTCAGTTTCTCCACTATGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MIRLET7BHG (400931)
Gene Description:
MIRLET7B host gene
Transcript:
RefSeq NM_207477.1 (NON-CURRENT)
Match location:
Position 1782 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000172723)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCAGTTTCTCCACTATGA
Hairpin Sequence:
5'-CCGG-GCCTCAGTTTCTCCACTATGA-CTCGAG-TCATAGTGGAGAAACTGAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCAGTTTCTCCACTATGACTCGAGTCATAGTGGAGAAACTGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCAGTTTCTCCACTATGACTCGAGTCATAGTGGAGAAACTGAGGC-3'

Other clones with same target sequence:

(none)