Construct: shRNA TRCN0000172781

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017991.3-2710s1c1
DNA Barcode:
GATCACTACACTGAGCCCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KANSL3 (55683)
Gene Description:
KAT8 regulatory NSL complex subunit 3
Transcript:
RefSeq NM_017991.3 (NON-CURRENT)
Match location:
Position 2710 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000172781)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCACTACACTGAGCCCTAT
Hairpin Sequence:
5'-CCGG-GATCACTACACTGAGCCCTAT-CTCGAG-ATAGGGCTCAGTGTAGTGATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCACTACACTGAGCCCTATCTCGAGATAGGGCTCAGTGTAGTGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCACTACACTGAGCCCTATCTCGAGATAGGGCTCAGTGTAGTGATC-3'

Other clones with same target sequence:

(none)