Construct: shRNA TRCN0000172861

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014040.2-323s1c1
DNA Barcode:
GAAGGTGCCTTCATCAACTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AAMDC (28971)
Gene Description:
adipogenesis associated Mth938 domain containing
Transcript:
RefSeq NM_014040.2 (NON-CURRENT)
Match location:
Position 323 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000172861)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGTGCCTTCATCAACTGT
Hairpin Sequence:
5'-CCGG-GAAGGTGCCTTCATCAACTGT-CTCGAG-ACAGTTGATGAAGGCACCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGTGCCTTCATCAACTGTCTCGAGACAGTTGATGAAGGCACCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGTGCCTTCATCAACTGTCTCGAGACAGTTGATGAAGGCACCTTC-3'

Other clones with same target sequence:

(none)