Construct: shRNA TRCN0000172968

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016623.3-924s1c1
DNA Barcode:
GCAGGCTCTTGCTAAACAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM49B (51571)
Gene Description:
family with sequence similarity 49 member B
Transcript:
RefSeq NM_016623.3 (NON-CURRENT)
Match location:
Position 924 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000172968)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGCTCTTGCTAAACAGTT
Hairpin Sequence:
5'-CCGG-GCAGGCTCTTGCTAAACAGTT-CTCGAG-AACTGTTTAGCAAGAGCCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGCTCTTGCTAAACAGTTCTCGAGAACTGTTTAGCAAGAGCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGCTCTTGCTAAACAGTTCTCGAGAACTGTTTAGCAAGAGCCTGC-3'

Other clones with same target sequence:

(none)