Construct: shRNA TRCN0000173024

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130809.2-443s1c1
DNA Barcode:
CCTTCGGTAATCCTCCTGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRRC1 (133619)
Gene Description:
proline rich coiled-coil 1
Transcript:
RefSeq NM_130809.2 (NON-CURRENT)
Match location:
Position 443 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173024)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCGGTAATCCTCCTGTAT
Hairpin Sequence:
5'-CCGG-CCTTCGGTAATCCTCCTGTAT-CTCGAG-ATACAGGAGGATTACCGAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCGGTAATCCTCCTGTATCTCGAGATACAGGAGGATTACCGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCGGTAATCCTCCTGTATCTCGAGATACAGGAGGATTACCGAAGG-3'

Other clones with same target sequence:

(none)