Construct: shRNA TRCN0000173036

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015397.1-2949s1c1
DNA Barcode:
CCTCCCTTGAACTGTCTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DCAF12 (25853)
Gene Description:
DDB1 and CUL4 associated factor 12
Transcript:
RefSeq NM_015397.1 (NON-CURRENT)
Match location:
Position 2949 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173036)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCCTTGAACTGTCTGTTT
Hairpin Sequence:
5'-CCGG-CCTCCCTTGAACTGTCTGTTT-CTCGAG-AAACAGACAGTTCAAGGGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCCTTGAACTGTCTGTTTCTCGAGAAACAGACAGTTCAAGGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCCTTGAACTGTCTGTTTCTCGAGAAACAGACAGTTCAAGGGAGG-3'

Other clones with same target sequence:

(none)