Construct: shRNA TRCN0000173180
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_133884.1-1119s1c1
- DNA Barcode:
- CTAGAGAAGAAGCCACGCATA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Gpn2 (100210)
- Gene Description:
- GPN-loop GTPase 2
- Transcript:
- RefSeq NM_133884.1 (NON-CURRENT)
- Match location:
- Position 1119 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000173180)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CTAGAGAAGAAGCCACGCATA
- Hairpin Sequence:
-
5'-CCGG-CTAGAGAAGAAGCCACGCATA-CTCGAG-TATGCGTGGCTTCTTCTCTAG-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTAGAGAAGAAGCCACGCATACTCGAGTATGCGTGGCTTCTTCTCTAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTAGAGAAGAAGCCACGCATACTCGAGTATGCGTGGCTTCTTCTCTAG-3'
Other clones with same target sequence:
(none)
