Construct: shRNA TRCN0000173191

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009862.1-1942s1c1
DNA Barcode:
CCTCTCAGTAATTGAGCTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdc45 (12544)
Gene Description:
cell division cycle 45
Transcript:
RefSeq NM_009862.1 (NON-CURRENT)
Match location:
Position 1942 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173191)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTCAGTAATTGAGCTGAA
Hairpin Sequence:
5'-CCGG-CCTCTCAGTAATTGAGCTGAA-CTCGAG-TTCAGCTCAATTACTGAGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTCAGTAATTGAGCTGAACTCGAGTTCAGCTCAATTACTGAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTCAGTAATTGAGCTGAACTCGAGTTCAGCTCAATTACTGAGAGG-3'

Other clones with same target sequence:

  1. TRCN0000279261