Construct: shRNA TRCN0000173192

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011286.2-3521s1c1
DNA Barcode:
CCATGACAAATCCAGAGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rph3a (19894)
Gene Description:
rabphilin 3A
Transcript:
RefSeq NM_011286.2 (NON-CURRENT)
Match location:
Position 3521 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173192)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGACAAATCCAGAGGAAT
Hairpin Sequence:
5'-CCGG-CCATGACAAATCCAGAGGAAT-CTCGAG-ATTCCTCTGGATTTGTCATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGACAAATCCAGAGGAATCTCGAGATTCCTCTGGATTTGTCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGACAAATCCAGAGGAATCTCGAGATTCCTCTGGATTTGTCATGG-3'

Other clones with same target sequence:

(none)