Construct: shRNA TRCN0000173217

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133906.3-1489s1c1
DNA Barcode:
CCATAGCTCAAATCTCATCCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zkscan1 (74570)
Gene Description:
zinc finger with KRAB and SCAN domains 1
Transcript:
RefSeq NM_133906.3 (NON-CURRENT)
Match location:
Position 1489 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173217)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATAGCTCAAATCTCATCCT
Hairpin Sequence:
5'-CCGG-CCATAGCTCAAATCTCATCCT-CTCGAG-AGGATGAGATTTGAGCTATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATAGCTCAAATCTCATCCTCTCGAGAGGATGAGATTTGAGCTATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATAGCTCAAATCTCATCCTCTCGAGAGGATGAGATTTGAGCTATGG-3'

Other clones with same target sequence:

(none)