Construct: shRNA TRCN0000173271

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144810.2-915s1c1
DNA Barcode:
CACCAATCAACTGCCTGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Klhdc8a (213417)
Gene Description:
kelch domain containing 8A
Transcript:
RefSeq NM_144810.2 (NON-CURRENT)
Match location:
Position 915 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173271)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCAATCAACTGCCTGTGAA
Hairpin Sequence:
5'-CCGG-CACCAATCAACTGCCTGTGAA-CTCGAG-TTCACAGGCAGTTGATTGGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCAATCAACTGCCTGTGAACTCGAGTTCACAGGCAGTTGATTGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCAATCAACTGCCTGTGAACTCGAGTTCACAGGCAGTTGATTGGTG-3'

Other clones with same target sequence:

(none)