Construct: shRNA TRCN0000173304

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172818.1-2573s1c1
DNA Barcode:
GCGGTTATACCAGAGTCCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ttll8 (239591)
Gene Description:
tubulin tyrosine ligase-like family, member 8
Transcript:
RefSeq NM_172818.1 (NON-CURRENT)
Match location:
Position 2573 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173304)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGTTATACCAGAGTCCAAT
Hairpin Sequence:
5'-CCGG-GCGGTTATACCAGAGTCCAAT-CTCGAG-ATTGGACTCTGGTATAACCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGTTATACCAGAGTCCAATCTCGAGATTGGACTCTGGTATAACCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGTTATACCAGAGTCCAATCTCGAGATTGGACTCTGGTATAACCGC-3'

Other clones with same target sequence:

(none)