Construct: shRNA TRCN0000173398

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198937.1-1491s1c1
DNA Barcode:
GCTCCTATAATTGGGAGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hn1l (52009)
Gene Description:
hematological and neurological expressed 1-like
Transcript:
RefSeq NM_198937.1 (NON-CURRENT)
Match location:
Position 1491 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173398)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCTATAATTGGGAGGAAA
Hairpin Sequence:
5'-CCGG-GCTCCTATAATTGGGAGGAAA-CTCGAG-TTTCCTCCCAATTATAGGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCTATAATTGGGAGGAAACTCGAGTTTCCTCCCAATTATAGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCTATAATTGGGAGGAAACTCGAGTTTCCTCCCAATTATAGGAGC-3'

Other clones with same target sequence:

(none)